A database of RNA aptamers
Example: RNA aptamer SELEX ATP-aptamer Structure
The systematic evolution of ligands by exponential enrichment (SELEX) is a powerful technique used for the in vitro selection of nucleic acid ligands, such as aptamers, against a wide range of targets including proteins, small molecules, and even complex systems.
For common improved methods in SELEX's evolution, please see the table below.
Detailed information
This section lists some of the improved methods that have been developed during SELEX's development.
SELEX Methods
| Name | Development | Description | Target | Examples |
|---|---|---|---|---|
| Conventional SELEX | 1990 | SELEX is a method used to select specific nucleic acid sequences, with specific functions from a large pool of random sequences. SELEX was developed in the early 1990s by Larry Gold and Jack Szostak as a solution to the challenge of isolating nucleic acid ligands capable of binding specific targets. | Proteins, small molecules, and even complex systems |
Malachite green (MG), DMHBI, DFHO, Oxazole thiazole blue (OTB), Tetramethylrhodamine (TMR), HBC, DFAME, NF-kappaB, Citrulline, Arginine, Flavin mononucleotide (FMN) , Flavin adenine dinucleotide (FAD), Cyanocobalamin (Vitamin B12), ATP, GTP, Tetracycline, Aminoglycoside antibiotic, Streptomycin, Theophylline, Tobramycin, Ribosomal protein S8, Mammalian translation initiation factor 4A (eIF4A) , Human IgG, Human thrombin, Yeast RNA polymerase II (Pol II) , Prion protein (PrPC), HTLV-1 arginine-rich Rex peptide, HIV-1 REV peptide, HIV Tat protein |
| Capillary electrophoresis-SELEX(CE-SELEX) | 2004 | CE-SELEX is a variation of the SELEX technique that integrates capillary electrophoresis as a separation method during the selection process. | Proteins, small molecules, and cells |
Immunoglobulin E (IgE), Human immunodeficiency virus reverse transcriptase (HIV-1 RT), Myotonic dystrophy kinase-related Cdc42-binding kinase (αMRCK), Bovine catalase |
| Magnetic-based SELEX(MB-SELEX) | 1997 | Magnetic bead-based SELEX is a variation of the SELEX technique that utilizes magnetic beads as a solid support matrix for the selection process. | Proteins, small molecules |
TO1-biotin, Transcription repressor TetR, HEWL (lysozyme), G-protein-coupled receptor kinase 2 (GRK2) |
| Cell-SELEX | 2006 | Cell-SELEX, also known as cell-based systematic evolution of ligands by exponential enrichment, is a technique derived from the SELEX method. It enables the selection of aptamers against cell surface molecules, receptors, or whole cells. | Cells and membrane proteins |
Human glutamate carboxypeptidase II (GCPII), Small cell lung cancer, Mouse tumor endothelial cells, Human hepatocarcinoma, Colorectal cancer |
| In vivo SELEX | In vivo SELEX-1: 1993 In vivo SELEX-2: 2010 |
In vivo SELEX is an extension of the SELEX method that aims to generate aptamers within a living organism, providing a more realistic selection pressure. | Biomolecules, cells, and tissues |
HIV-1, Rous sarcoma virus, Hepatic colon cancer metastases, Capillary endothelia and parenchyma in brain |
| One round-SELEX | 2007a 2007b 2008 2012a 2012b |
One round-SELEX is an evolution of the traditional SELEX methodology, designed to address some of the limitations and inefficiencies associated with multiple rounds of selection by reducing the number of rounds required to identify high-affinity aptamers, thereby saving time and resources. | Small molecules, proteins and cells |
Vaccinia virus, Ovalbumin, Botulinum neurotoxin, Kallikrein-related peptidase 6 (KLK6) |
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