Timeline

<div class="title">
  <h3><a href="https://pubmed.ncbi.nlm.nih.gov/7510417/" target="_blank" style="color:#520049">1994</a></h3>
</div>
<div class="body">
  <p>Aptamers are isolated out for the first time<sup>[<a href="#ref1" style="color:#520049">1</a>]</sup></p>
</div>

<div class="title">
  <h3><a href="https://pubmed.ncbi.nlm.nih.gov/9224568/" target="_blank" style="color:#520049">1997</a></h3>
</div>
<div class="body">
  <p>First demonstrated that the aptamer could be linked to stem II of a self-cleaving hammerhead ribozyme<sup>[<a href="#ref2" style="color:#520049">2</a>]</sup></p>
</div>

Description

In 1994, Polisky, B. et al. used SELEX to screen for high-affinity RNA aptamers with theophylline-binding solution structures. In 1997, NMR techniques visualised the interaction of RNA structures in the ligand-binding site.

References

[1] High-resolution molecular discrimination by RNA.
Jenison, R. D., Gill, S. C., Pardi, A., & Polisky, B.
Science (New York, N.Y.), 263(5152), 1425–1429. (1994)

[2] Rational design of allosteric ribozymes.
Tang, J., & Breaker, R. R.
Chemistry & biology, 4(6), 453–459. (1997)