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10G-1 aptamer
Timeline
An aptamer called 10G-1, which binds specifically to the NS3 protease, was selected for the first time by in vitro genetic-selection strategy[1]
Nishikawa, S. et al. isolated aptamers that inhibited the activity of NS3 protease and helicase[2]
Nishikawa, S. et al. isolated RNA aptamers that bind specifically to the NS3 protease active site in the truncated polypeptide ΔNS3[3]
The binding sites of NS3 protease domain and aptamer were analyzed by alanine scanning mutagenesis[4]
Nishikawa, S. et al. constructed a G9 aptamer expression system in cultured cells, using the cytomegarovirus enhancer + chicken beta-actin globin (CAG) promoter[5]
Nishikawa, S. et al. designed a new aptamer called NEO-III-14U to inhibit both protease and helicase activity[6]
Kao, C. C. et al. showed that RNAs can bind directly to the active site cleft of the NS3 protease domain (NS3P) and inhibit proteolysis of peptide substrates. RNAs that are less apt to form intramolecular structures have a stronger inhibitory activity than RNAs with more stable base paired regions[7]
Description
In 1997, Nishikawa, S. and colleagues used an in vitro genetic-selection strategy to isolate high-affinity RNA aptamers that bind to the NS3 protein, especially to its protease domain. Starting from a RNA pool that had a random sequence core of 12-18 nucleotides, aptamers that bind specifically to the NS3 protein were selected after 10 rounds of selection and amplification. A single aptamer, 10G-1, was found predominantly (71%) in the selected pool. This aptamer could bind to the NS3 protein with a binding constant of 650 nM and inhibit the proteolytic activity in vitro. By phosphate-modification-interference analysis we showed that the phosphate residues that are critical for the binding of 10G-1 to NS3 lie within the selected regions of the aptamer and that binding involves electrostatic contacts with the phosphates of regions G28-U34 and A47-A55[1].
SELEX
In 1997, Nishikawa, S. and colleagues used existing research to design the method required for the in vitro genetic-selection. Starting from a RNA pool that had a random sequence core of 12-18 nucleotides, aptamers that bind specifically to the NS3 protein were selected after 10 rounds of selection and amplification. A single aptamer, 10G-1, was found predominantly (71%) in the selected pool[1].
Detailed information are accessible on SELEX page.
Structure
10G-1 was the aptamer sequence mainly studied in the article, which had a high affinity with HCV NS3 protease. The 2D structure of the figure is based on the article by ribodraw tool to draw. The 10G-1 aptamer was named by Nishikawa, S. et al. in the article[1].
5'-GGGAACUCGAUGAAGCGAAUUCUGUUGGCGAACUGUACGCAAGUACACUGGAUGACAGCCUAUCUAUCGGAUCCACG-3'
Ligand information
SELEX ligand
Hepatitis C virus NS3 protein is a serine protease which has a trypsin-like fold. The non-structural (NS) protein NS3 is one of the NS proteins involved in replication of the HCV genome. The action of NS3 protease (NS3P), which resides in the N-terminal one-third of the NS3 protein, then yields all remaining non-structural proteins.-----From Pfam
UniProt ID: uniquely identifies protein sequences in the UniProt database, a resource for protein information.
Pfam: a widely recognised database of protein families and domains.
GenBank: maintained by NCBI(National Center for Biotechnology Information), is a database of nucleotide sequences from various organisms, vital for genetic and molecular biology research.
Mass: an intrinsic property of a body.
| Name | Uniprot ID | Pfam | Mass | Protein sequence | PDB ID | GenBank |
|---|---|---|---|---|---|---|
| HCV NS3 protease | B2Y2M9 | PF02907 | 19.15 kDa |
......
APITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRS (Residues 1027-1207)
|
3KF2 | ABY67662.1 |
Some isolated sequences bind to the affinity of the protein[1].
| Name | Sequence | Ligand | Affinity |
|---|---|---|---|
| 10G-1 aptamer | 5'-GGGAACUCGAUGAAGCGAAUUCUGUUGGCGAACUGUACGCAAGUACACUGGAUGACAGCCUAUCUAUCGGAUCCACG-3' | HCV NS3 protease | 650 nM |
| 10G-2 aptamer | 5'-GGGAACUCGAUGAAGCGAAUUUGUCGGGAUGUACGCAAGUACAUGUUCUCACAGGCCUAUCUAUCGGAUCCACG-3' | HCV NS3 protease | NA |
| 10G-8 aptamer | 5'-GGGAACUCGAUGAAGCGAAUUUGUCGGGAUGUACGCAAGUACUUGGUUCCUACAGGCCUAUCUAUCGGAUCCACG-3' | HCV NS3 protease | NA |
Similar compound(s)
We used the Dail server website to compare the structural similarities of ligand proteins, and chose the top 10 in terms of similarity for presentation.
Dail server website: a network service for comparing protein structures in 3D. Dali compares them against those in the Protein Data Bank (PDB).
Z-score: a standard score that is converted from an original score. The list of neighbours is sorted by Z-score. Similarities with a Z-score lower than 2 are spurious.
RMSD: (Root Mean Square Deviation) is used to measure the degree to which atoms deviate from the alignment position.
PDB: PDB ID+ chain name.
| PDB | Z-socre | RMSD (Å) | Description |
|---|---|---|---|
| 3KF2-B | 37.5 | 0 | Polyprotein |
| 2OIN-B | 35.7 | 0.3 | Polyprotein |
| 2O8M-A | 35.7 | 0.3 | Protease |
| 3LON-A | 35.5 | 0.3 | Genome polyprotein |
| 2OC0-A | 35.5 | 0.3 | Hepatitis C virus |
| 2A4R-A | 35.3 | 0.3 | NS3 protease/helicase |
| 2QV1-B | 35.3 | 0.3 | NS3 |
| 3KN2-A | 35.3 | 0.3 | HCV NS3 protease domain |
| 2A4Q-A | 35.3 | 0.3 | NS3 protease/helicase |
| 3EYD-A | 35.3 | 0.3 | HCV NS3 |
References
[1] Selection of RNA aptamers that bind specifically to the NS3 protease of hepatitis C virus.Urvil, P. T., Kakiuchi, N., Zhou, D. M., Shimotohno, K., Kumar, P. K., & Nishikawa, S.
European journal of biochemistry, 248(1), 130–138. (1997)
[2] Isolation of RNA aptamers specific to the NS3 protein of hepatitis C virus from a pool of completely random RNA.
Kumar, P. K., Machida, K., Urvil, P. T., Kakiuchi, N., Vishnuvardhan, D., Shimotohno, K., Taira, K., & Nishikawa, S.
Virology, 237(2), 270–282. (1997)
[3] Isolation and characterization of RNA aptamers specific for the hepatitis C virus nonstructural protein 3 protease.
Fukuda, K., Vishnuvardhan, D., Sekiya, S., Hwang, J., Kakiuchi, N., Taira, K., Shimotohno, K., Kumar, P. K., & Nishikawa, S.
European journal of biochemistry, 267(12), 3685–3694. (2000)
[4] The RNA aptamer-binding site of hepatitis C virus NS3 protease.
Hwang, J., Fauzi, H., Fukuda, K., Sekiya, S., Kakiuchi, N., Shimotohno, K., Taira, K., Kusakabe, I., & Nishikawa, S.
Biochemical and biophysical research communications, 279(2), 557–562. (2000)
[5] Inhibition of HCV NS3 protease by RNA aptamers in cells.
Nishikawa, F., Kakiuchi, N., Funaji, K., Fukuda, K., Sekiya, S., & Nishikawa, S.
Nucleic acids research, 31(7), 1935–1943. (2003)
[6] An RNA ligand inhibits hepatitis C virus NS3 protease and helicase activities.
Fukuda, K., Umehara, T., Sekiya, S., Kunio, K., Hasegawa, T., & Nishikawa, S.
Biochemical and biophysical research communications, 325(3), 670–675. (2004)
[7] RNA binding by the NS3 protease of the hepatitis C virus.
Vaughan, R., Li, Y., Fan, B., Ranjith-Kumar, C. T., & Kao, C. C.
Virus research, 169(1), 80–90. (2012)