Clone 5 aptamer

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Description

In 2001,Yu J et al. screened an RNA aptamer targeting Sialyl Lewis X (sLeX) and determined its affinity and specificity by surface plasmon resonance technique, and found that its affinity was about 1000-fold higher than that of the original RNA library, and verified the aptamer's application in inhibiting adhesion of sLeX-expressing HL60 cells to E- and P-selectin by static in vitro experiments[1].


SELEX

Jaehoon Yu et al. screened RNA aptamers against Sialyl Lewis X (sLeX), mainly by screening Clone 5 RNA aptamers from a random RNA library containing 70 random nucleotides by an in vitro screening program, and after 17 rounds of screening, a final aptamer sequence was obtained that had a high affinity and high specificity. The affinity of the aptamer for sLeX was determined using surface plasmon resonance, and the KD value for Clone 5 was 0.085 nM[1].
Detailed information are accessible on SELEX page.



Structure

The sequence and secondary prediction structure of the aptamer will be shown here, here we used ribodraw to complete the figure. The 2D structure of the figures is based on the prediction results of the RNA fold website by ribodraw tool to draw. We used the minimum free energy (MFE) structure. Clone 5 aptamer binding to Sialyl Lewis X (sLeX).

5'-CCGUAAUACGACUCACUAUAGGGGAGCUCGGUACCGAAUUCAAGGYACUCUGUGCUUGUCGAUGUGYAUUGAUGGCACUUUCGAGUCAACGAGUUGACAGRACAAGUAGUCAAGCUUUGCAGAGAGGAUCCUU-3'

drawing


Ligand information

SELEX ligand

Sialyl-Lewis X (sLeX) is a salivarylated polytetrasaccharide, an endogenous antigen.Sialyl-Lewis X is a high-affinity ligand for the selectins E-, P-, and L-.Sialyl-Lewis X binds to ELAM-1 and CD62 and has the ability to inhibit CD62-mediated neutrophil recruitment at sites of inflammation. Sialyl-Lewis X binds to ELAM-1 and CD62 and has the ability to inhibit CD62-mediated neutrophil recruitment at sites of inflammation.-----From ChEBI
Name Molecular Formula MW CAS Solubility PubChem Drug ID
Sialyl Lewis X (sLeX) C31H52N2O23 820.7 g/mol NA NA 44279132 NA
drawing

Similar compound

We used the PubChem database to screen compounds that were more similar to Sialyl Lewis X (sLeX), and selected the most similar compounds for display. For those without CAS numbers, we will supplement them with Pubchem CID.

Named CAS Pubchem CID Structure
6-Sialyllactosamine 64364-50-7 125128 drawing
3'-Sialyllactose 35890-38-1 123914 drawing
Hyaluronan NA 3084049 drawing
3'-beta-Sialyl-beta-lactose 64839-32-3 448469 drawing
G(M1)-Oligosaccharide 85373-04-2 196569 drawing
NeuAcalpha2-6Galbeta NA 5289369 drawing


References

[1] In vitro selection of the RNA aptamer against the Sialyl Lewis X and its inhibition of the cell adhesion.
Jeong S, Eom T, Kim S, Lee S, Yu J
Biochemical and biophysical research communications. 2001 Feb 16;281(1):237-43. (2001)