tMG RNA aptamer

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Timeline

2002

Hwang B et al. libraries containing extra random nucleotides extended to the 3′ end of previously selected RNA sequences[2]

2003

Hwang B et al. isolated as described to select RNA aptamers against mAb198[1]

Description

 In 2003, Hwang B and Han K had used the methodology which is in vitro transcription of synthetic DNA templates, radiolabeled, and isolated as described to select RNA aptamers against mAb198 . The aptamers, tMG RNA , binds with high affinity to specifically recognize mAb198[1].

SELEX

In 2003, Hwang B and Han K selected RNA aptamer by the methodology that full-length RNA aptamer with 2P-deoxy-2P-£uoropyrimidines (Ambion) previously selected against mAb198 or its truncated form (fMG RNA or tMG RNA, respectively) was synthesized by in vitro transcription of synthetic DNA templates, radiolabeled, and isolated[1].
Detailed information are accessible on SELEX page.



Structure

The 2D structure of the figure is based on the article by ribodraw tool to draw[1].

5'GGGCCGGAGGUUAGCUUGCCCAUGGCAAGCAGGGCGCCACGGACCC3'

 drawing


Ligand information

SELEX ligand

Clinical symptoms of EAMG in rats engendered by passive transfer of mAb198.-----from paper

Name Uniprot ID Pfam MW Amino acids sequences PDB Gene ID
mAb198(rat monoclonal antibody) NA NA 1.88 kDa PMTLPENYFSERPYH 2JRV 2JRV_A

The aptamer bind to the affinity of the protein.

Name Sequence Ligand Affinity
tMG RNA 5'GGGCCGGAGGUUAGCUUGCCCAUGGCAAGCAGGGCGCCACGGACCC3' mAb198(rat monoclonal antibody) 30 nM
drawing


References

[1] Prevention of passively transferred experimental autoimmune myasthenia gravis by an in vitro selected RNA aptamer.
Hwang B, Han K, Lee SW.
FEBS Lett. 548(1-3):85-9. (2003)
[2] Improvement of RNA aptamer activity against myasthenic autoantibodies by extended sequence selection.
Hwang B, Lee SW.
Biochem Biophys Res Commun. 290(2):656-62. (2002)